Abstract: The rural revitalization strategy puts forward the overall requirement of effective governance. The collaborative governance of multiple subjects is of great significance to the modernization of rural governance. Based on the collaborative governance theory, this paper takes Lianzhang Village in Yingde City, Qingyuan City, Guangdong Province as a case to explore the interactive mechanism and inherent law of multiple subjects such as grassroots governments, Village Party Branch Committee and Village Committee, enterprises and villagers in rural governance. The study found that: First, Lianzhang Village promotes top-down macro support and bottom-up grassroots autonomy in a coordinated way to achieve quality improvement and transformation of rural governance. Second, two committees in Lianzhang Village serve as a bridge between the grassroots government, enterprises and villagers, and consolidate the interdependence of multiple subjects through resource integration, policy communication and information sharing. Finally, Lianzhang Village establishes an interest balance mechanism at levels of economic, institutional and civilization construction, taking account of public, collective and individual demands. In conclusion, this paper reveals the inherent law of collaborative governance of multiple subjects in rural areas, and provides theoretical support and practical reference for other villages in northern Guangdong.