Abstract: With the comprehensive implementation of the rural revitalization strategy, the protection and utilization of traditional villages along the Yellow River have ushered in new opportunities. From the perspective of symbiosis theory, this paper analyzes existing issues in these villages, including low correlation among symbiotic unit resource elements, a simplistic symbiotic interface medium, and weak positive incentives in the symbiotic environment. There is an urgent need to establish an effective symbiosis model that transforms competition into cooperation. Focusing on symbiotic units, interfaces, and environments, this study takes Laoniuwan Area of the Yellow River as a case study. First, it identifies cultural, industrial, and spatial elements within symbiotic units and examines current challenges. Second, it analyzes symbiotic interfaces, addressing both hard and soft mediums.Third, it investigates the symbiotic environment by evaluating policy, awareness, and developmental factors. Finally,optimization strategies are proposed through three dimensions: coordinating symbiotic units, strengthening interfaces,and improving environments. This approach aims to construct a regional linkage framework for traditional villages along the Yellow River, fostering mutual benefit and achieving rural revitalization goals. The study provides insights for developing culturally and industrially integrated villages with regional characteristics.